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Bicarbonate solution (1M)

Internal Solution-ID: S3547

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 84 g 84 999.917
Double distilled water 1000 mL - -
1 The solution was dispensed in the required portions (40 mL per 1 L medium). The head space of the bottles was flushed with CO2. Saturation of CO2 in the solution was achieved by repeated flushing and shaking of the bottles. Finally, the bottles were sealed with rubber stoppers and aluminum crimps and autoclaved.
1712 AROMATOLEUM MEDIUM