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Internal Solution-ID: S3790

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Isobutyric acid 0.5 ml - -
2-Methylbutyric acid 0.5 ml - -
Isovaleric acid 0.5 ml - -
Valeric acid 0.5 ml - -
NaOH
(0.1 N) 		100.0 ml - -
1 Add volatile fatty acids to about 70-80 ml of NaOH solution and mix. Add NaOH solution to bring final volume to 100 ml. If not used the same day, filter-sterilize and store at 4C.
J144 MODIFIED NOS MEDIUM