Main sol. J159
Internal Solution-ID: S3812
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000.0 | ml | - | - | |
| NaCl | 40.0 | g | 40 | 684.463 | |
|
Yeast extract
(BD-Difco) |
2.0 | g | 2 | - | |
|
Sulfur
(powder) |
10.0 | g | 10 | 311.818 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| 1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, adjust pH to 5.8 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 5.8, if necessary. | |||||
Media with this solution
| J159 | THERMOCOCCUS CELER MEDIUM |