Main sol. J206
Internal Solution-ID: S3871
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt base solution | 1000.0 | ml | - | - | |
| Trace minerals | 10.0 | ml | - | - | |
| Citric acid | 5.0 | mg | 0.005 | 0.026 | |
|
Tryptone
(BD-Difco) |
6.0 | g | 5.941 | 83.576 | |
|
Sulfur
(powder) |
6.0 | g | 5.941 | 185.238 | |
| Na2S x 9 H2O | 0.5 | g | 0.495 | 2.061 | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| 1 Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20). | |||||
Media with this solution
| J206 | JCM MEDIUM No. 206 |