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Main sol. J298

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Internal Solution-ID: S4002

Compound Amount Unit Conc. [g/L] Conc. [mM]
Glycerol 2.5 g 1250 13573.7
Sodium pyruvate 2.5 g 1250 11359.5
K2HPO4 0.5 g 250 1435.33
(NH4)2SO4 1.0 g 500 3783.87
KCl 4.0 g 2000 26827.6
NaCl 200.0 g 100000 1711160
MgSO4 x 7 H2O 20.0 g 10000 40571.9
Trace metal solution 2.0 ml - -
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15.
2 For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco).
J298 HALOSIMPLEX MEDIUM