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Main sol. 196

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Internal Solution-ID: S402

Equipment needed: Gassing station Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 961 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
1 First dissolve 2.7 g FeCl3 x 6 H2O in distilled water and adjust the pH to 7.0 with 1 N NaOH (about 3 ml). Add and dissolve remaining salts of solution A and sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 min, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved separately under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be adjusted to 7.2.
2 Note: For transfers use 5 - 10% (v/v) inoculum.
196 DESULFOCAPSA SULFEXIGENS MEDIUM