1
Use Medium No. 365 supplemented with 0.2 g/L (final) yeast extract and 1.0 g/L NaHCO3, and omitted sodium acetate. Filter-sterilize NaHCO3 as an 8% aqueous solution, and add to the medium. Fill the inoculated bottles with H2-CO2 (4:1), and pressurize to 100 kPa.
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