Main sol. J402
Internal Solution-ID: S4130
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casamino acids
(BD-Difco) |
5.0 | g | - | - | |
| Yeast extract | 5.0 | g | - | - | |
| Tris base | 12.1 | g | - | - | |
| KCl | 2.0 | g | - | - | |
| MgCl2 x 6 H2O | 20.0 | g | - | - | |
| CaCl2 x 2 H2O | 0.2 | g | - | - | |
| NaCl | 200.0 | g | - | - | |
| 1 Add components to distilled water and bring volume to 1.0 L. | |||||
| 2 Adjust pH to 7.4. | |||||
| 3 For preparation of solid medium, add 20.0 g/L agar. | |||||
| 4 Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma). | |||||
Media with this solution
| J402 | HALOCOCCUS DOMBOWSKII MEDIUM |