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Main sol. J440

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Internal Solution-ID: S4187

Equipment needed: Autoclave
1 Use Medium No. 371 supplemented with 30.0 g/L (final) NaCl. Prior to inoculation, add an additional 3% Na2S x 9H2O solution (neutralized, autoclaved and stored under a N2 atmosphere, 4 ml per liter of the medium) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture.
J440 TB2 MEDIUM