Main sol. J902
Internal Solution-ID: S4901
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| NH4Cl | 0.25 | g | 0.243 | 4.538 | |
| 1 Mix components, adjust pH to 5.5 and autoclave. After cooling, add aseptically the following solutions (filter-sterilized): | |||||
|
NaHCO3
(8%) |
5.00 | ml | - | - | |
|
Na2S2O3 x 5 H2O
(10%) |
15.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| 2 Distribute the medium into sterile culture vessels under a N2-CO2-O2 (76:19:5, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the culture vessels to 50 kPa with the same gas mixture. | |||||
Media with this solution
| J902 | MJ MEDIUM FOR MICROAEROPHILIC AUTOTROPHS |