Main sol. J975
Internal Solution-ID: S4999
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.00 | g | 100 | 1711.16 | |
| Trisodium citrate | 3.00 | g | 3 | 11.625 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| KCl | 2.00 | g | 2 | 26.828 | |
| MnCl2 x 4 H2O | 0.36 | mg | 3.6e-4 | 0.002 | |
| FeSO4 x 7 H2O | 0.05 | g | 0.05 | 0.18 | |
|
Yeast extract
(BD-Difco) |
10.00 | g | 10 | - | |
| 1 Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved): | |||||
|
Na2CO3
(w/v, 10%) |
30.00 | ml | 3 | 28.305 | |
| 2 Check the final pH to be around 9.0. | |||||
Media with this solution
| J975 | SALINIVIBRIO SHARMENSIS MEDIUM |