Main sol. J1273
Internal Solution-ID: S5415
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaNO3 | 120.0 | mg | 0.12 | 1.412 | |
| K2HPO4 | 5.0 | mg | 0.005 | 0.029 | |
| Vitamin B12 | 1.0 | μg | - | - | |
| Biotin | 1.0 | μg | - | - | |
| Thiamine HCl | 100.0 | μg | - | - | |
| Fe(III)-EDTA | 259.0 | μg | - | - | |
| Mn(II) x EDTA | 332.0 | μg | - | - | |
| Tris(hydroxymethyl)aminomethane | 1.0 | g | 1 | 8.255 | |
|
LB Broth
, Lennox
(BD-Difco) |
0.1 | g | 0.1 | - | |
| Sea water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave. | |||||
| 2 Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water. | |||||
Media with this solution
| J1273 | 0.01% LB SEAWATER MEDIUM |