Main sol. J1279
Internal Solution-ID: S5423
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Wolfe's mineral solution | 1000.00 | ml | - | - | |
| Trace minerals | 10.00 | ml | - | - | |
| MES | 1.95 | g | 1.875 | 9.604 | |
| NaHCO3 | 0.83 | g | 0.798 | 9.5 | |
| Sodium acetate | 1.36 | g | 1.308 | 15.942 | |
| Sodium pyruvate | 1.10 | g | 1.058 | 9.612 | |
| 1 Mix components thoroughly and adjust pH 6.2. Autoclave under a N2 atmosphere. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
Yeast extract
(w/v, 10%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J1279 | MIZ14 MEDIUM |