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Main sol. 855

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Internal Solution-ID: S5661

Equipment needed: Hungate tubes Autoclave Gassing station Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 30 ml - -
Solution E 1 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B and C are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Adjust pH of final medium to 7.2, if necessary.
2 For aerobic growth prepare medium under air. Omit carbonate and KNO3. 3,5-Dihydroxybenzoic acid or Na-benzoate as substrate can be replaced with 10 mM Na-malate. Adjust medium pH to 7.2.
855 THAUERA AROMATICA MEDIUM (ANAEROB)