Main sol. 301
Internal Solution-ID: S568
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Wilkins-Chalgren-Anaerobe-Broth
(Oxoid) |
1000.0 | ml | - | - | |
| L-Cysteine | 0.3 | g | 0.3 | 2.476 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
|
Sterile serum
(horse, bovine or lamb) |
50.0 | ml | - | - | |
| 1 Wilkins-Chalgren-Anaerobe-Broth (Oxoid) is supplemented with 0.3 g/l of L-cysteine and 1.0 mg/l of resazurin. Prepare the medium under 100% nitrogen gas. To the autoclaved medium add 50 ml/l of sterile serum (horse, bovine or lamb). | |||||
Media with this solution
| 301 | FALCIVIBRIO MEDIUM |