Main sol. 1790
Internal Solution-ID: S5948
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Brain heart infusion | 9.260 | g | - | - | |
Trypticase soy broth | 7.500 | g | - | - | |
Yeast extract | 2.500 | g | - | - | |
NaNO3 | 0.426 | g | - | - | |
K2HPO4 | 1.250 | g | - | - | |
1 Adjust pH to 7.0. | |||||
2 Bring to boil, cool down under N2CO2. | |||||
Cysteine HCl | 0.250 | g | - | - | |
3 Fill under N2CO2 in hungate tubes (10 ml) | |||||
4 Autoclave at 121 °C for 15 min. | |||||
Haemin solution | 0.050 | ml | - | - | |
Vitamin K3 solution | 0.050 | ml | - | - | |
Fetal bovine serum | 0.500 | ml | - | - | |
5 pressurize with 1 bar H2 |
1790 | Mucispirillum Spezial |