MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 194b

Download this solution as

CSV JSON PDF

Internal Solution-ID: S5987

Equipment needed: Gassing station Filter Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 962 ml - -
Solution B 30 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
1 Dissolve ingredients of solution A and adjust pH to 6.8, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to make it anoxic. Distribute solution under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution D is autoclaved under 100% N2 gas. Solutions B to D are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.
194b OXALOPHAGUS MEDIUM