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Main sol. 194b

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Internal Solution-ID: S5987

Equipment needed: Gassing station Filter Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 962 ml - -
Solution B 30 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
1 Dissolve ingredients of solution A and adjust pH to 6.8, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to make it anoxic. Distribute solution under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution D is autoclaved under 100% N2 gas. Solutions B to D are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.
194b OXALOPHAGUS MEDIUM