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Mail sol. C4

Internal Solution-ID: S5995

Compound Amount Unit Conc. [g/L] Conc. [mM]
Tricine 0.50 g 0.5 2.791
Extra salts 3.75 ml - -
Vitamin solution 2.50 ml - -
SE1 (Soil Extract 1) 25.00 ml - -
1 Make up to 1 Litre with filtered natural seawater. Adjust pH 7.6 - 7.8 to with 1M NaOH or 1M HCl, prior to autoclaving. Autoclave at 15 psi for 15 minutes.
2 Alternatively, use: Distilled water to 1 Litre and “Ultramarine Synthetica” sea salts 33.60g
C4 ASW