Mail sol. C19
Internal Solution-ID: S6010
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tris | 1 | g | 1 | 8.255 | |
| NaCl | 30 | g | 30 | 513.347 | |
|
MgSO4 x 7 H2O
(24.4 g/100 ml stock solution) |
10 | ml | - | - | |
|
KCl
(6 g/100 ml stock solution) |
10 | ml | - | - | |
|
NaNO3
(10 g/100 ml stock solution) |
10 | ml | - | - | |
|
CaCl2 x 2 H2O
(3 g/100 ml stock solution) |
10 | ml | - | - | |
|
KH2PO4
(0.5 g/100 ml stock solution) |
10 | ml | 23.4 | 171.951 | |
|
NH4Cl
(2.67 g/100 ml stock solution) |
1 | ml | 1.527 | 28.555 | |
| Trace Elements (PIV) | 6 | ml | - | - | |
| 1 Once elements are dissolved autoclave at 15 psi for 15 minutes. | |||||
| Vitamin B1 solution | 1 | ml | - | - | |
| Vitamin B12 solution | 1 | ml | - | - | |
| SE2 (Soil Extract 2) | 30 | ml | - | - | |
| 2 Make up to 1 litre with distilled water and adjust to pH 8.0 with1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes. | |||||
| 3 For brackish organisms, take 15 g of NaCl instead of 30 g (BW/MASM). | |||||
Media with this solution
| C19 | BW/MASM |