Mail sol. C99
Internal Solution-ID: S6090
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Soil
(air-dried, sieved, garden loam) |
trace | g | - | - | |
| 1 Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.) Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0. | |||||
Media with this solution
| C99 | S/W |