ASW (Artificial Seawater)
Internal Solution-ID: S6125
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tricine | 0.50 | g | 0.5 | 2.791 | |
| Extra salts | 3.75 | ml | - | - | |
|
Vitamin solution
(may be stored frozen at -20°C) |
2.50 | ml | - | - | |
| SE1 (Soil Extract 1) | 25.00 | ml | - | - | |
|
Natural sea water
(filtered) |
1000.00 | ml | - | - | |
| 1 Make up to 1 litre with filtered natural sea water. Adjust the pH to 7.6 – 7.8 with 1N NaOH or 1N HCl. Autoclave at 15 psi. | |||||
| 2 Alternatively use "Ultramarine Synthetica" sea salts 33.6 g and make up to 1 litre with distilled water. Adjust pH as above | |||||
Media with this solution
| C11 | ASW:SES |