MY75S (Malt & Yeast Extract 75% Seawater Agar)
Internal Solution-ID: S6197
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Natural sea water
(filtered) |
750.0 | ml | - | - | |
| Deionized water | 250.0 | ml | - | - | |
|
Malt extract
(Oxoid L39) |
0.1 | g | 0.1 | - | |
|
Yeast extract
(Oxoid L21) |
0.1 | g | 0.1 | - | |
|
Agar
(Oxoid L11) |
15.0 | g | 15 | - | |
| 1 Thoroughly disperse the agar in cold 75% seawater. Bring to the boil, stirring continuously. During heating add the other constituents. Transfer the molten agar to suitable vessels and sterilise at 15 psi for 15 minutes. If available a bench top agar maker is preferable to making this medium. The constant stirring during sterilisation minimises flocculation. | |||||
Media with this solution
| C54 | MErds/MY75S |