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Mail sol. C100

Internal Solution-ID: S6238

Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried, sieved, garden loam)
trace g - -
(NH4)MgPO4 0.01 g 0.01 -
1 Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.) Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0.
2 S/W is prepared as above, but approximately 0.01 g ammonium magnesium phosphate is placed into the base of the test tube before adding the soil and water.
C100 S/W + AMP