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Main sol. 337

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Internal Solution-ID: S643

Equipment needed: Gassing station Hungate tubes Filter Autoclave Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 921 ml - -
Solution B 50 ml - -
Solution C 1 ml - -
Solution D 25 ml - -
Solution E 10 ml - -
Solution F 1 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 6.4 and dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions D, E and F are autoclaved separately under 100% N2 gas. To complete the medium add appropriate amounts of solutions B - F to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.4 - 6.8, if necessary.
2 Note: Rumen fluid may be replaced by supplementing the medium with 200 µg/ml of crude egg yolk phosphatidylcholine (SIGMA, type IX-E).
337 SYNTROPHOCOCCUS SUCROMUTANS MEDIUM