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Main sol. 347a

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Internal Solution-ID: S679

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 921 ml - -
Solution B 30 ml - -
Solution C 1 ml - -
Solution D 25 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
1 Dissolve ingredients of solution A, adjust pH to 7.0, then sparge with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust pH of complete medium to 7.2, if necessary.
2 Note: Solutions D and F lead to a white precipitate which makes the medium very cloudy. Alternatively, solutions D and F can be replaced with 10 ml/l of a 14% (w/v) solution of Na-Caproate, which does not precipitate.
347a SYNTROPHOMONAS ZEHNDERI MEDIUM