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Main sol. 428

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Internal Solution-ID: S858

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 50 ml - -
Solution B 855 ml - -
Solution C 20 ml - -
Solution D 30 ml - -
Solution E 50 ml - -
Standard vitamin solution 5 ml - -
1 H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367).
2 Solutions A, B, C and D are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then mixed aseptically with filter-sterilized solution E (at 50°C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment.
428 HETEROTROPHIC MEDIUM H3P