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Main sol. 441

Internal Solution-ID: S890

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 953 ml - -
Solution B 50 ml - -
Standard vitamin solution 5 ml - -
1 Sterilize solution A separately at 121°C for 15 min., cool to 50°C and then mix aseptically with filter-sterilized solution B and 5.0 ml of filter-sterilized standard vitamin solution (see medium 428).
2 RBA is an ammonium-free medium which has successfully been used for the isolation, growth and purity check of a broad spectrum of nitrogen fixing bacteria (Ref. 3363). For microaerophilic nitrogen-fixing bacteria use semisolid medium with 0.3% end concentration of agar and incubate the liquid cultures under 10% (v/v) air and 90% (v/v) N2.