Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 457 | 1000.00 | ml | - | - | |
|
Biphenyl
(10 g/l in ethanol) |
0.25 | g/l | 0.25 | 1.621 | |
| 1 Prepare medium 457. Make a stock solution of 10 g/l biphenyl in ethanol and filter sterilize it using a cellulose filter membrane. Add an aliquot of this stock solution to a sterile flask so that the final concentration will be 0.25 g/l biphenyl, and let the ethanol evaporate. Add sterile medium to the crystal-layered flask. Alternatively, add biphenyl crystals directly to the liquid medium or to the lid of inversely incubated petri dishes containing 457-agar medium. | |||||
Media with this solution
| 457d | MEDIUM WITH BIPHENYL |