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Main sol. 1200

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Internal Solution-ID: S2389

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 1.00 ml - -
Solution C 0.20 ml - -
Solution D ("red mix") 0.20 ml - -
Solution E (electron acceptor) 0.05 ml - -
Solution F: Vitamin solution according to Wolin and coauthors 0.20 ml - -
1 Prepare solutions A, B, and D separately and anaerobically under 80% N2 + 20% CO2 and autoclave. Filter-sterilize solutions C, E and F and outgas with N2. After cooling, add about 1 ml solution B per 10 ml solution A, by this way adjusting pH to 7.2. Add 0.2 ml solution C, 0.2 ml solution D, 0.2 ml solution F and 0.05 ml solution E, each per 10 ml solution A.
1200 ANAEROMYXOBACTER-MEDIUM