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Main sol. J480

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Internal Solution-ID: S4234

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.348 g 29 166.498
KH2PO4 0.227 g 18.917 139.005
NH4Cl 0.500 g 41.667 778.962
MgSO4 x 7 H2O 0.500 g 41.667 169.05
CaCl2 x 2 H2O 0.250 g 20.833 141.709
NaCl 2.250 g 187.5 3208.42
Trace vitamins 10.000 ml - -
Trace element solution 1.000 ml - -
Se/W solution 1.000 ml - -
NaHCO3 4.000 g 333.333 3967.92
Resazurin 1.000 mg 0.083 0.364
Dithiothreitol 0.300 g 25 162.022
Mannitol 3.600 g 300 1646.81
Yeast extract 0.500 g 41.667 -
Casitone
(BD-Difco) 		0.500 g 41.667 -
1 Dissolve components, except NaHCO3, dithiothreitol and mannitol, in 900 ml distilled water and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately autoclave 3% (w/v) dithiothreitol solution and 1 M mannitol solution under a N2 atmosphere. Add filter-sterilized 5% NaHCO3 [stored under a N2-CO2 (4:1, v/v) atmosphere] to the medium. Prior to inoculation, add the sterile dithiothreitol and mannitol solutions.
J480 MODIFIED SPOROMUSA MEDIUM