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Medium 1611 in Hungate tube

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Internal Solution-ID: S6268

Equipment needed: Autoclave Hungate tubes Filter
1 Prepare the Medium (see Main sol. 1611). Distribute under N2 into Hungate tubes (10 ml):
Compound Amount Unit Conc. [g/L] Conc. [mM]
Main sol. 1611 10.0 ml - -
2 Autoclave. Then filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube:
Vitamin solution
(filter-sterilized) 		0.1 ml - -
1611 YCFA MEDIUM (modified)