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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Ampicillin sodium salt
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1436 PIRELLULA MEDIUM (M31PY)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 18 | 53.519 |
| N-Acetylglucosamine | 2 | 9.041 |
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Peptone | 0.25 | - |
| Yeast extract | 0.25 | - |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Cycloheximid | 0.2 | 0.711 |
| Ampicillin sodium salt | 0.2 | 0.539 |
| Na2HPO4 x 2 H2O | 0.1 | 0.704 |
| MgCl2 x 6 H2O | 0.1 | 0.492 |
| CaCl2 x 2 H2O | 0.067 | 0.454 |
| ZnSO4 x 7 H2O | 0.001 | 0.004 |
| FeSO4 x 7 H2O | 5.0e-4 | 0.002 |
| Na-EDTA | 2.5e-4 | 7.4e-4 |
| Pyridoxine hydrochloride | 2.0e-4 | 9.7e-4 |
| (NH4)6Mo7O24 x 4 H2O | 1.8e-4 | - |
| MnSO4 x H2O | 1.5e-4 | 9.1e-4 |
| Nicotinamide | 1.0e-4 | 8.2e-4 |
| p-Aminobenzoic acid | 1.0e-4 | 7.3e-4 |
| Thiamine-HCl x 2 H2O | 1.0e-4 | 2.7e-4 |
| Riboflavin | 1.0e-4 | 2.7e-4 |
| Calcium pantothenate | 1.0e-4 | 2.1e-4 |
| Biotin | 4.0e-5 | 1.6e-4 |
| Folic acid | 4.0e-5 | 9.1e-5 |
| CuSO4 x 5 H2O | 3.9e-5 | 1.6e-4 |
| Co(NO3)2 x 6 H2O | 2.5e-5 | 8.5e-5 |
| Na2B4O7 x 10 H2O | 1.8e-5 | - |
| Vitamin B12 | 2.0e-6 | 1.5e-6 |
| Tris-HCl buffer | - | 4.75 |
| Distilled water | - | - |
Solutions:
- 50 ml "Metals 44"
- 950 ml Solution 1
- 50 ml Solution 2
- 20 ml Hutner's salts
- 10 ml Vitamin solution
1437 ALKALIPHILES PIRELLULA MEDIUM (M1PY)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Gelrite | 9 | - |
| CaCO3 | 5 | 49.955 |
| N-Acetylglucosamine | 2 | 9.041 |
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Peptone | 0.25 | - |
| Yeast extract | 0.25 | - |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Cycloheximid | 0.2 | 0.711 |
| Ampicillin sodium salt | 0.2 | 0.539 |
| Na2HPO4 x H2O | 0.1 | 0.704 |
| CaCl2 x 2 H2O | 0.067 | 0.454 |
| ZnSO4 x 7 H2O | 0.001 | 0.004 |
| FeSO4 x 7 H2O | 5.0e-4 | 0.002 |
| Na-EDTA | 2.5e-4 | 7.4e-4 |
| Pyridoxine hydrochloride | 2.0e-4 | 9.7e-4 |
| (NH4)6Mo7O24 x 4 H2O | 1.8e-4 | - |
| MnSO4 x H2O | 1.5e-4 | 9.1e-4 |
| Nicotinamide | 1.0e-4 | 8.2e-4 |
| p-Aminobenzoic acid | 1.0e-4 | 7.3e-4 |
| Thiamine-HCl x 2 H2O | 1.0e-4 | 2.7e-4 |
| Riboflavin | 1.0e-4 | 2.7e-4 |
| Calcium pantothenate | 1.0e-4 | 2.1e-4 |
| Biotin | 4.0e-5 | 1.6e-4 |
| Folic acid | 4.0e-5 | 9.1e-5 |
| CuSO4 x 5 H2O | 3.9e-5 | 1.6e-4 |
| Co(NO3)2 x 6 H2O | 2.5e-5 | 8.5e-5 |
| Na2B4O7 x 10 H2O | 1.8e-5 | - |
| Vitamin B12 | 2.0e-6 | 1.5e-6 |
| Distilled water | - | - |
Solutions:
- 50 ml "Metals 44"
- 950 ml Solution 1
- 50 ml Solution 2
- 20 ml Hutner's salts
- 10 ml Vitamin solution