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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Ce(NO3)3 x 6 H2O
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1738 METHYLOMONAS MEDIUM (WSC)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| HEPES | 2.383 | 9.998 |
| NaOH | 0.295 | 7.376 |
| NaCl | 0.263 | 4.5 |
| Na2HPO4 | 0.142 | 1 |
| MgSO4 x 7 H2O | 0.123 | 0.499 |
| KCl | 0.112 | 1.502 |
| CaCl2 x 2 H2O | 0.044 | 0.299 |
| NaNO3 | 0.043 | 0.5 |
| SrCl2 x 6 H2O | 0.007 | 0.026 |
| HCl | 0.002 | 0.05 |
| FeSO4 x 7 H2O | 0.001 | 0.004 |
| MnCl2 x 4 H2O | 2.5e-4 | 0.001 |
| CuSO4 x 5 H2O | 2.5e-4 | 0.001 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Na2MoO4 x 2 H2O | 5.8e-5 | 2.4e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| NiCl2 x 6 H2O | 5.0e-5 | 3.9e-4 |
| 4-Aminobenzoic Acid | 5.0e-5 | 3.6e-4 |
| Thioctic acid | 5.0e-5 | 2.4e-4 |
| Thiamine-HCl x 2 H2O | 5.0e-5 | 1.3e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium D-Pantothenate | 5.0e-5 | - |
| ZnSO4 x 7 H2O | 3.5e-5 | 1.2e-4 |
| CoCl2 | 3.0e-5 | 2.3e-4 |
| Ce(NO3)3 x 6 H2O | 3.0e-5 | - |
| Show more | ||
Solutions:
- 50 ml Solution 1 (20X Basal Salts Solution)
- 10 ml Solution 2 (Wolfe's Vitamin Solution)
- 0.5 ml Solution 3 (Acidic Trace Elements Solution)
- 0.2 ml Solution 4 (Alkaline Trace Elements Solution)
- 25 ml Solution 5 (40X Buffer Solution)
1739 METHYLOMONAS MEDIUM (SURF)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| HEPES | 2.383 | 9.998 |
| NaCl | 1.55 | 26.523 |
| NaOH | 0.295 | 7.376 |
| Na2SO4 | 0.188 | 1.32 |
| Na2HPO4 | 0.142 | 1 |
| MgSO4 x 7 H2O | 0.123 | 0.499 |
| CaCl2 x 2 H2O | 0.044 | 0.299 |
| NaNO3 | 0.043 | 0.5 |
| KCl | 0.026 | 0.349 |
| HCl | 0.002 | 0.05 |
| FeSO4 x 7 H2O | 0.001 | 0.004 |
| MnCl2 x 4 H2O | 2.5e-4 | 0.001 |
| CuSO4 x 5 H2O | 2.5e-4 | 0.001 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Na2MoO4 x 2 H2O | 5.8e-5 | 2.4e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| NiCl2 x 6 H2O | 5.0e-5 | 3.9e-4 |
| 4-Aminobenzoic Acid | 5.0e-5 | 3.6e-4 |
| Thioctic acid | 5.0e-5 | 2.4e-4 |
| Thiamine-HCl x 2 H2O | 5.0e-5 | 1.3e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium D-Pantothenate | 5.0e-5 | - |
| ZnSO4 x 7 H2O | 3.5e-5 | 1.2e-4 |
| CoCl2 | 3.0e-5 | 2.3e-4 |
| Ce(NO3)3 x 6 H2O | 3.0e-5 | - |
| Show more | ||
Solutions:
- 50 ml Solution 1 (20X Basal Salts Solution)
- 10 ml Solution 2 (Wolfe's Vitamin Solution)
- 0.5 ml Solution 3 (Acidic Trace Elements Solution)
- 0.2 ml Solution 4 (Alkaline Trace Elements Solution)
- 25 ml Solution 5 (40X Buffer Solution)
1741 METHYLOCYSTIS MEDIUM (NLS)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KNO3 | 1 | 9.891 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Na2HPO4 | 0.285 | 2.004 |
| KH2PO4 | 0.272 | 1.999 |
| CaCl2 x 2 H2O | 0.135 | 0.918 |
| HCl | 7.3e-4 | 0.02 |
| FeSO4 x 7 H2O | 4.2e-4 | 0.002 |
| NaOH | 2.0e-4 | 0.005 |
| Na2MoO4 x 2 H2O | 1.5e-4 | 6.0e-4 |
| MnCl2 x 4 H2O | 1.0e-4 | 5.1e-4 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| CuSO4 x 5 H2O | 1.0e-4 | 4.0e-4 |
| Na2SeO4 | 5.5e-5 | 2.9e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| 4-Aminobenzoic Acid | 5.0e-5 | 3.6e-4 |
| Thioctic acid | 5.0e-5 | 2.4e-4 |
| Thiamine-HCl x 2 H2O | 5.0e-5 | 1.3e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium D-Pantothenate | 5.0e-5 | - |
| Na2WO4 x 2 H2O | 2.5e-5 | 7.6e-5 |
| NiCl2 x 6 H2O | 2.0e-5 | 1.5e-4 |
| D-Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| ZnSO4 x 7 H2O | 1.4e-5 | 4.9e-5 |
| CoCl2 | 1.2e-5 | 9.2e-5 |
| La(NO3)3 x 6 H2O | 1.2e-5 | - |
| Ce(NO3)3 x 6 H2O | 1.2e-5 | - |
| Show more | ||
Solutions:
- 50 ml Solution A
- 50 ml Solution B
- 0.2 ml Solution C (Acidic Trace Elements Solution)
- 0.5 ml Solution D (Alkaline Trace Elements Solution)
- 10 ml Solution E (Wolfe's Vitamin Solution)