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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Colloidal chitin
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1681 Mineral salt medium with colloidal chitin
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Polypeptone | 10 | - |
| Colloidal chitin | 5 | - |
| NaHCO3 | 3.52 | 41.901 |
| Yeast extract | 1 | - |
| KH2PO4 | 0.4 | 2.939 |
| K2HPO4 | 0.4 | 2.297 |
| NH4Cl | 0.25 | 4.674 |
| MgCl2 x 6 H2O | 0.2 | 0.984 |
| MgSO4 x 7 H2O | 0.03 | 0.122 |
| Nitrilotriacetic acid | 0.015 | 0.078 |
| NaCl | 0.01 | 0.171 |
| MnSO4 x H2O | 0.005 | 0.03 |
| CoSO4 x 7 H2O | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 0.002 | 0.006 |
| CaCl2 x 2 H2O | 1.0e-3 | 0.007 |
| FeSO4 x 7 H2O | 1.0e-3 | 0.004 |
| Sodium resazurin | 1.0e-3 | - |
| NiCl2 x 6 H2O | 3.0e-4 | 0.002 |
| AlK(SO4)2 x 12 H2O | 2.0e-4 | 4.2e-4 |
| H3BO3 | 1.0e-4 | 0.002 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Na2MoO4 x 2 H2O | 1.0e-4 | 4.1e-4 |
| CuSO4 x 5 H2O | 1.0e-4 | 4.0e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 5.0e-5 | 2.4e-4 |
| Thiamine HCl | 5.0e-5 | 1.5e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium D-(+)-pantothenate | 5.0e-5 | - |
| Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| Na2WO4 x 2 H2O | 4.0e-6 | 1.2e-5 |
| Na2SeO3 x 5 H2O | 3.0e-6 | 1.1e-5 |
| Vitamin B12 | 1.0e-6 | 7.4e-7 |
| Distilled water | - | - |
Solutions:
- 50 ml Solution A
- 50 ml Solution B
- 10 ml Modified Wolin's mineral solution
- 10 ml Wolin's vitamin solution
J1274 BM7CO MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 6 | - |
| Na2CO3 | 4 | 37.74 |
| K2HPO4 | 2.9 | 16.65 |
| Urea | 2.1 | 34.967 |
| KH2PO4 | 1.5 | 11.022 |
| L-Cysteine HCl x H2O | 0.5 | - |
| CaCl2 x 2 H2O | 0.018 | 0.119 |
| MgCl2 x 6 H2O | 0.005 | 0.025 |
| Resazurin | 5.0e-4 | 0.002 |
| FeSO4 x 7 H2O | 6.2e-5 | 2.2e-4 |
| Distilled water | - | - |
| Colloidal chitin | - | - |
Solutions:
- 0.2 ml Mineral solution