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The medium composition is currently in its alpha status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Searched compounds: 2 - 10 g/l Glucose MgCl2

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591 HALOBACTEROIDES/HALOINCOLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
NaCl 174.825 2991.4
Glucose 4.995 27.726
NaHCO3 1.499 17.838
NH4Cl 0.33 6.163
KCl 0.33 4.422
MgCl2 0.33 3.463
CaCl2 0.33 2.97
KH2PO4 0.33 2.423
Na2S x 9 H2O 0.5 2.08
HCl 0.003 0.087
Resazurin 0.002 0.009
FeCl2 x 4 H2O 0.001 0.008
CoCl2 x 6 H2O 1.9e-4 8.0e-4
ZnCl2 7.0e-5 5.1e-4
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Nicotinic acid 5.0e-5 4.1e-4
p-aminobenzoic acid 5.0e-5 3.6e-4
Lipoic acid 5.0e-5 2.4e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
Thiamine-HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
D-Ca-pantothenate 5.0e-5 1.0e-4
NiCl2 x 6 H2O 2.4e-5 1.0e-4
H3BO3 6.0e-6 9.7e-5
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Vitamin B12 1.0e-6 6.3e-7
Peptone 4.995 -
Solutions:
  • 10 ml Vitamin solution
  • 1 ml Trace element solution SL-10
Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
MgCl2 100 1050.31
NaCl 5 85.554
Glucose 5 27.754
Agar 15 -
Casein peptone, tryptic digest 10 -
Yeast extract 5 -