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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Magnesium acetate
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1761 PFENNIG'S MEDIUM III
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 1.5 | 17.856 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| Ammonium chloride | 0.4 | 7.478 |
| Na2S x 9 H2O | 0.385 | 1.604 |
| KCl | 0.34 | 4.561 |
| KH2PO4 | 0.34 | 2.498 |
| Ammonium acetate | 0.25 | 3.243 |
| Magnesium acetate | 0.25 | 1.756 |
| CaCl2 x 2 H2O | 0.25 | 1.701 |
| Na2-EDTA | 0.003 | 0.009 |
| Resazurin | 0.002 | 0.01 |
| FeSO4 x 7 H2O | 0.001 | 0.004 |
| H3BO3 | 3.0e-4 | 0.005 |
| CoCl2 x 6 H2O | 1.9e-4 | 0.001 |
| MnCl2 x 2 H2O | 5.0e-5 | - |
| ZnCl2 | 4.2e-5 | 3.1e-4 |
| NiCl2 x 6 H2O | 2.4e-5 | 1.9e-4 |
| Vitamin B12 | 2.0e-5 | 1.5e-5 |
| Na2MoO4 x 2 H2O | 1.8e-5 | 7.4e-5 |
| CuCl2 x 2 H2O | 2.0e-6 | 1.2e-5 |
| Distilled water | - | - |
| H2O | - | - |
Solutions:
- 46 ml Solution A
- 2.6 ml Solution B
- 5 ml Solution C
- 45 ml Solution D
- 2.6 ml Solution E
- 0.1 ml Solution F
- 1 ml Solution G
- 1 ml Trace element solution SL-12 B