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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: TAPS sodium salt
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1206 CALDANAEROVIRGA MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| D-Xylose | 2.967 | 19.765 |
| TAPS sodium salt | 2.374 | - |
| NaHCO3 | 1.484 | 17.661 |
| Yeast extract | 0.989 | - |
| NH4Cl | 0.495 | 9.246 |
| (NH4)2SO4 | 0.495 | 3.743 |
| KH2PO4 | 0.267 | 1.962 |
| L-Cysteine HCl x H2O | 0.247 | 1.408 |
| Na2S x 9 H2O | 0.247 | 1.03 |
| MgSO4 x 7 H2O | 0.03 | 0.12 |
| Nitrilotriacetic acid | 0.015 | 0.078 |
| NaCl | 0.01 | 0.169 |
| MgCl2 x 6 H2O | 0.01 | 0.049 |
| MnSO4 x H2O | 0.005 | 0.029 |
| CoSO4 x 7 H2O | 0.002 | 0.011 |
| ZnSO4 x 7 H2O | 0.002 | 0.006 |
| CaCl2 x 2 H2O | 9.9e-4 | 0.007 |
| FeSO4 x 7 H2O | 9.9e-4 | 0.004 |
| Sodium resazurin | 4.9e-4 | 0.002 |
| NiCl2 x 6 H2O | 3.0e-4 | 0.002 |
| AlK(SO4)2 x 12 H2O | 2.0e-4 | 4.2e-4 |
| H3BO3 | 9.9e-5 | 0.002 |
| Pyridoxine hydrochloride | 9.9e-5 | 4.8e-4 |
| Na2MoO4 x 2 H2O | 9.9e-5 | 4.1e-4 |
| CuSO4 x 5 H2O | 9.9e-5 | 4.0e-4 |
| Nicotinic acid | 4.9e-5 | 4.0e-4 |
| p-Aminobenzoic acid | 4.9e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 4.9e-5 | 2.4e-4 |
| Calcium D-(+)-pantothenate | 4.9e-5 | 2.1e-4 |
| Thiamine HCl | 4.9e-5 | 1.5e-4 |
| Riboflavin | 4.9e-5 | 1.3e-4 |
| Biotin | 2.0e-5 | 8.1e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| Na2WO4 x 2 H2O | 4.0e-6 | 1.2e-5 |
| Na2SeO3 x 5 H2O | 3.0e-6 | 1.1e-5 |
| Vitamin B12 | 9.9e-7 | 7.3e-7 |
| Distilled water | - | - |
Solutions:
- 10 ml Modified Wolin's mineral solution
- 1 ml Wolin's vitamin solution (10x)
Gas phase:
- 100 % N2