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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Tris buffer
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1091 HALOQUADRATUM WALSBYI MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 195 | 3336.76 |
| MgSO4 x 7 H2O | 50 | 202.86 |
| MgCl2 x 6 H2O | 35 | 172.157 |
| KCl | 5 | 67.069 |
| Tris buffer | 2.423 | 20 |
| NaNO3 | 1 | 11.765 |
| Sodium pyruvate | 1 | 9.088 |
| CaCl2 x 2 H2O | 0.5 | 3.401 |
| NaHCO3 | 0.25 | 2.976 |
| KH2PO4 | 0.05 | 0.367 |
| Yeast extract | 0.05 | - |
| NH4Cl | 0.03 | 0.561 |
| Distilled water | - | - |
1242 ALGORIPHAGUS ALKALIPHILUS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Tris buffer | 12.114 | 100 |
| Glucose | 1 | 5.551 |
| Proteose peptone no. 3 | 1 | - |
| Casamino acids | 1 | - |
| Yeast extract | 1 | - |
| K2HPO4 | 0.6 | 3.445 |
| NaNO3 | 0.345 | 4.053 |
| Na2HPO4 | 0.056 | 0.391 |
| KNO3 | 0.052 | 0.509 |
| Pyruvic acid | 0.05 | 0.568 |
| Nitrilotriacetic acid | 0.05 | 0.262 |
| MgSO4 x 7 H2O | 0.05 | 0.203 |
| NaCl | 0.04 | 0.684 |
| CaSO4 x 2 H2O | 0.03 | 0.174 |
| MnSO4 x H2O | 0.001 | 0.007 |
| H3BO3 | 2.5e-4 | 0.004 |
| ZnSO4 x 7 H2O | 2.5e-4 | 8.7e-4 |
| CoCl2 x 6 H2O | 2.3e-5 | 1.8e-4 |
| Na2MoO4 x 2 H2O | 1.3e-5 | 5.2e-5 |
| CuSO4 x 5 H2O | 1.3e-5 | 5.0e-5 |
| Distilled water | - | - |
Solutions:
- 50 ml Mineralsolution 1
- 5 ml Traceelements Solution