1058a .
THIOHALOSPIRA HALOPHILA MEDIUM
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Equipment needed:
Autoclave
Gassing station
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 233.00 | g | ||
|
|
K2HPO4 | 1.50 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
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Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | ||
|
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CaCl2 x 2 H2O | 0.05 | g | ||
|
|
MgCl2 x 6 H2O | 0.40 | g | ||
|
|
Na2S2O3 x 5 H2O | 5.00 | g | ||
|
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NaHCO3 | 2.50 | g | ||
|
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Seven vitamins solution | 1.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under air atmosphere in Erlenmeyer flasks to 10% of volume, close with screw caps and autoclave. Add trace elements, calcium chloride, magnesium chloride, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.0 - 7.2, if necessary. Incubate with shaking. | |||||
| 2 Note: Use at least 10% (v/v) as inoculum. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.20 | g | ||
|
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ZnSO4 x 7 H2O | 0.10 | g | ||
|
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MnCl2 x 4 H2O | 0.03 | g | ||
|
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H3BO3 | 0.03 | g | ||
|
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CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.03 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
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Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |