1059 .
GEOALKALIBACTER MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
9.0 - 9.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.5 | g | ||
|
|
KCl | 0.2 | g | ||
|
|
MgCl2 x 6 H2O | 0.1 | g | ||
|
|
KH2PO4 | 0.2 | g | ||
|
|
NaCl | 1.0 | g | ||
|
|
Trace element solution SL-10 | 1.0 | ml | ||
|
|
Selenite-tungstate solution | 1.0 | ml | ||
|
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Na2CO3 | 3.0 | g | ||
|
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NaHCO3 | 10.0 | g | ||
|
|
Sulfur
(powdered) |
10.0 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Na-acetate | 2.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients (ecxept carbonate, hydrogencarbonate, sulfur, yeast extract and acetate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.0 - 9.2, and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by autoclaving at 110°C for 20 min. Add yeast extract and acetate from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
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ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |