DSMZ 105 . GLUCONOBACTER OXYDANS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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* Modified for Komagataeibacter sucrofermentans DSM 15973

With 30 g CaCO3

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 100 g 100 555.062
Yeast extract 10 g 10 -
CaCO3 20 g 20 199.82
Distilled water 1000 ml - -
Agar , for solid medium 15 g/l 15 44.599
CaCO3* 30 g - -
1 Adjust pH to 6.8.
2 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. In liquid culture the calcium carbonate also forms a sediment that clears as acid is produced.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 37
Strains with modifications: 2
Komagataeibacter sucrofermentans DSM 15973

  30.0 g CaCO3

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Glucose 100 555.062
CaCO3 30 299.73
Agar 15 44.599
Yeast extract 10 -
Distilled water - -

Cultivation metadata from 37 strains BacDive

pH 2 - 4 2 strains
phyla 1 37 strains
oxygen obligate aerobe 3 strains
oxygen aerobe 2 strains
temperature mesophilic 37 strains