DSMZ 1076a . SP4 MEDIUM WITH GLUCOSE

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Equipment needed: Autoclave Filter

Final pH: 7.4

Select a strain (optional):

ml
Compound Amount Unit
Medium 1076 517.0 ml
Glucose
(10 %)
5.2 ml
1 Use medium 1076, but add 5.2 ml 10% glucose (filter-sterilized).

Main sol. 1076 #

515
Compound Amount Unit
Tryptone 5.00 g
Peptone 2.65 g
PPLO broth 1.75 g
Distilled water 375.00 ml
1 Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
2 Add aseptically:
Fetal bovine serum
(heat-inactivated)
90.00 ml
CMRL 1066
(10x concentrated; GIBCO)
25.00 ml
Yeast extract
(20%, autoclaved)
17.50 ml
Yeast extract
(20%, autoclaved)
5.00 ml
Phenol red
(1%, filter-sterilized)
1.00 ml
L-Glutamine
(200 mM; GIBCO)
1.70 ml