1081a .
HORIKOSHI-1 MEDIUM WITH 10% NaCl
What is this page?
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 1081 | 1000 | ml | ||
|
|
NaCl | 100 | g | ||
| 1 Use medium 1081 (HORIKOSHI-1 MEDIUM), but add 100 g NaCl. | |||||
Main sol. 1081 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10.0 | g | ||
|
|
Polypeptone | 5.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
K2HPO4 | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 900.0 | ml | ||
|
|
Na2CO3
(10 %) |
100.0 | ml | ||
| 1 After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0. | |||||