1101a .
DESULFONATRONUM ZHILINAE MEDIUM
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Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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NaCl | 25.00 | g | ||
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K2HPO4 | 0.50 | g | ||
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Yeast extract | 0.20 | g | ||
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Na2SO4 | 3.00 | g | ||
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NaHCO3 | 1.85 | g | ||
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Na2CO3 | 1.20 | g | ||
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MgCl2 x 6 H2O | 0.10 | g | ||
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Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | ||
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Selenite-tungstate solution | 1.00 | ml | ||
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Na-L-lactate | 2.20 | g | ||
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Seven vitamins solution | 1.00 | ml | ||
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Na2S x 9 H2O | 0.50 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except carbonates, magnesium chloride, trace elements, lactate, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then add and dissolve carbonates, adjust pH to 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride, trace elements, lactate, vitamins and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 9.0 - 9.5, if necessary. | |||||
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
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FeSO4 x 7 H2O | 2.20 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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MnCl2 x 4 H2O | 0.03 | g | ||
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H3BO3 | 0.03 | g | ||
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CoCl2 x 6 H2O | 0.20 | g | ||
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CuCl2 x 2 H2O | 0.03 | g | ||
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2MoO4 x 2 H2O | 0.03 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
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Na2SeO3 x 5 H2O | 3.0 | mg |
|
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Na2WO4 x 2 H2O | 4.0 | mg |
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Distilled water | 1000.0 | ml |
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
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D-(+)-biotin | 20 | mg |
|
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Nicotinic acid | 200 | mg |
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Calcium pantothenate | 100 | mg |
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Pyridoxine hydrochloride | 300 | mg |
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Thiamine-HCl x 2 H2O | 200 | mg |
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Distilled water | 1000 | ml |