DSMZ 1113 . LEPTOSPIRA-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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* Modified for Leptonema illini DSM 21528

Semisolid medium; growth occurs in typical rings.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Leptospira Medium Base EMJH
(Difco)
2.3 g 2.3 -
Agarose 1.5 g 1.5 2.379
Water 900.0 ml - -
Leptospira Enrichment EMJH 100.0 ml - -
1 Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C.

Metadata

Last modified: 01.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 14
Strains with modifications: 13
Leptonema illini DSM 21528

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Leptospira Medium Base EMJH 2.3 -
Agarose 1.5 2.379
Water - -
Leptospira Enrichment EMJH - -

Cultivation metadata from 14 strains BacDive

phyla 2 47 strains
oxygen aerobe 3 strains
oxygen anaerobe 1 strain
temperature mesophilic 16 strains
temperature psychrophilic 1 strain