1121 .
MMJS MEDIUM (modified)
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Equipment needed:
Autoclave
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 20.00 | g | ||
|
|
K2HPO4 | 0.09 | g | ||
|
|
KH2PO4 | 0.07 | g | ||
|
|
CaCl2 x 2 H2O | 0.80 | g | ||
|
|
NH4Cl | 1.25 | g | ||
|
|
MgSO4 x 7 H2O | 4.00 | g | ||
|
|
MgCl2 x 6 H2O | 3.00 | g | ||
|
|
KCl | 0.33 | g | ||
|
|
Fe(III) citrate | 0.01 | g | ||
|
|
FeSO4 x 7 H2O | 0.01 | g | ||
|
|
Na2S2O3 x 5 H2O | 3.00 | g | ||
|
|
Sulfur
(powdered) |
3.00 | g | ||
|
|
Trace mineral solution | 10.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Finally, the mix gas (79%N2, 20%CO2, 1%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm. | |||||
|
|
Vitamin solution | 1.00 | ml/l | ||
|
|
NaHCO3 | 0.20 | % | ||
| 2 The pH of the medium is adjusted with NaOH to 6.8. Steam medium for 3 hours on each of 3 successive days. The separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%N2, 20%CO2) is purged for 5 min. | |||||
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g |
|
|
MnSO4 x n H2O | 0.50 | g |
|
|
CoSO4 x 7 H2O | 0.50 | g |
|
|
ZnSO4 x 7 H2O | 0.18 | g |
|
|
CuSO4 x 5 H2O | 0.01 | g |
|
|
AlK(SO4)2 x 12 H2O | 0.02 | g |
|
|
H3BO3 | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 1.00 | mg |
|
|
SrCl2 x 6 H2O | 0.01 | g |
|
|
NaBr | 0.01 | g |
|
|
KI | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.10 | g |
|
|
Na2SeO3 x 5 H2O | 0.10 | g |
|
|
Fe2(SO4)3 x n H2O | 1.00 | g |
|
|
H2WO4 | 0.10 | g |
|
|
Distilled water | 1000.00 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
D-Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |