1135 .
HD-MEDIUM, 1:10 diluted, modified
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Selected strain: Edaphobacter aggregans DSM 19364
This strain uses the standard medium without modifications.
Growth conditions: 20 °C
Equipment needed:
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A , adjust if required | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
| 1 Adjust to pH 5.5 (with NaOh/HCl) | |||||
| 2 Sterilize separately. For solid medium combine solution A with solution B at 50°C. | |||||
| 3 Liquid and solid medium possible. Incubation at 20°C, 4 days. | |||||
Solution A #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Casein peptone | 0.50 | g |
|
|
Glucose | 0.10 | g |
|
|
Yeast extract | 0.25 | g |
|
|
MES
(2-[N-Morpholino]ethane sulfonic acid) |
1.95 | g |
|
|
Double distilled water
(for liquid medium) |
1000.00 | ml |
|
|
Double distilled water
(for solid medium) |
500.00 | ml |
Solution B #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Agar
(washed agar with double distilled water) |
1.5 | % |
|
|
Double distilled water | 500.0 | ml |