DSMZ 1173 . MODIFIED MEDIUM 514

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Equipment needed:

Final pH: 7.4 - 7.8

Select a strain (optional):

ml
Compound Amount Unit
Bacto peptone 10.00 g
Yeast extract
(Bacto )
5.00 g
Fe(III) citrate 0.10 g
NaCl 39.45 g
MgCl2
(anhydrous)
5.90 g
Na2SO4 3.24 g
CaCl2 1.80 g
KCl 0.55 g
NaHCO3 0.16 g
KBr 0.08 g
SrCl2 34.00 mg
H3BO3 22.00 mg
Na-silicate 4.00 mg
NaF 2.40 mg
(NH4)NO3 1.60 mg
Na2HPO4 8.00 mg
Distilled water 1000.00 ml
1 Final pH should be 7.6 ± 0.2 at 25°C.