1183
1183 .
ROSEICYCLUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Photocaulis sulfatitolerans DSM 113471
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
aerobic
28 °C
pH 8.00
Main sol. 1183 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.3 | g | 0.3 | 4.024 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| MgSO4 | 2.0 | g | 2 | 16.615 | |
| Na2SO4 | 15.0 | g | 15 | 105.604 | |
| Malic acid | 1.0 | g | 1 | 7.458 | |
| Na acetate | 1.0 | g | 1 | 12.19 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Trace elements solution | 2.0 | ml | - | - | |
| Vitamin solution | 2.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Add to the autoclaved medium the following, from sterile stock solutions | |||||
|
K2HPO4
(10%) |
3.0 | ml | 0.3 | 1.722 | |
|
NaHCO3
(10%) |
5.0 | ml | 0.5 | 5.952 | |
|
CaCl2
(10%) |
0.5 | ml | 0.05 | 0.451 | |
| 2 pH is about 5.9 before autoclaving. | |||||
| 3 The pH of the final medium should be 8.0-8.5. | |||||
Solution occurs in 1
other media (first defined in Medium 1183).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nicotinic acid | 400.0 | mg | 0.4 | 3.249 | |
| Thiamine | 400.0 | mg | 0.4 | 1.507 | |
| Biotin | 80.0 | mg | 0.08 | 0.327 | |
| Vitamin B12 | 0.5 | mg | 5.0e-4 | 3.7e-4 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter sterilise | |||||
Solution occurs in 1
other media (first defined in Medium 1183).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeSO4 x 7 H2O | 300 | mg | 0.3 | 1.079 | |
| ZnSO4 x 7 H2O | 5 | mg | 0.005 | 0.017 | |
| MnCl2 x 4 H2O | 3 | mg | 0.003 | 0.015 | |
| H3BO3 | 2 | mg | 0.002 | 0.032 | |
| CoCl2 x 6 H2O | 1 | mg | 1.0e-3 | 0.008 | |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 3 | mg | 0.003 | 0.023 | |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust the pH to 3.0 with HCl and filter sterilise, store at 4°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.0 - 8.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Photocaulis sulfatitolerans DSM 113471
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2SO4 | 15 | 105.604 |
| MgSO4 | 2 | 16.615 |
| Na acetate | 1 | 12.19 |
| Malic acid | 1 | 7.458 |
| Yeast extract | 1 | - |
| Show more | ||
