1198
1198 .
Vero B4 Medium
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1198 #
ml
Equipment needed: Filter
Centrifuge
| 1 Cultivation of Vero B4 (ACC 33) cells | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| IMDM-Medium | 45.0 | ml | - | - | |
| Fetal bovine serum | 5.0 | ml | - | - | |
|
Aminoacids
(100 x) |
0.5 | ml | - | - | |
| 2 Filter-sterilize (0.2 µm) and keep no longer than 4 weeks. Store at room temperature to facilitate detection of contamination. | |||||
| 3 Prepare a 25 cm2 flask and seed cells according to standard protocols (see DSMZ catalogue for ACC 33). Incubate at 37°C plus 5% CO2. When a confluent layer has formed, infection can be carried out. | |||||
| 4 Infect cells with 1 ml of Rickettsia stock solution (thawed quickly to 37°C). | |||||
| 5 Centrifuge for 1 h onto the cell layer at 1600 rpm at 20°C. | |||||
| 6 Incubate at 28°C. Up to 90% of Vero cells should be infected after 15 days. Specific PCRs can be performed to verify the presence of rickettsiae. Ultimately, in situ-hybridization can be used to visualize them. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Centrifuge, Filter |
| Edit in Medium builder | |
Gas composition
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Aminoacids | - | - |
| Fetal bovine serum | - | - |
| IMDM-Medium | - | - |
